Multinuclear NMR studies of intracellular cations in perfused hypertensive rat kidney.

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Multinuclear NMR studies of intracellular cations in perfused hypertensive rat kidney.

We have investigated hypertension-associated alterations in intracellular cations in the kidney by measuring intracellular pH, free Mg2+, free Ca2+, and Na+ concentrations in perfused normotensive and hypertensive rat (8-14 weeks old) kidneys using 31P, 19F, and double quantum-filtered (DQ) 23Na NMR. The effects of both anoxia and ischemia on the 23Na DQ signal confirmed its ability to detect c...

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Multinuclear NMR studies of the Langendorff perfused rat heart.

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NMR studies of the effect of hyperglycemia on intracellular cations in rat kidney.

Alterations in intracellular cation concentrations in the kidney during hyperglycemia may play a role in a number of complications associated with diabetes mellitus such as renal hypertrophy and hypertension. In this study, we have investigated the effect of 25 mM glucose on intracellular pH and free Mg2+, free Ca2+, and Na+ concentrations in the perfused kidneys of Sprague-Dawley rats using 31...

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Multinuclear NMR Studies of Isolated Rabbit Hearts

The hypothesis that substrate availability can alter contractile function in reperfused myocardium after global ischemia was investigated in this study. Isolated rabbit hearts were placed in a dual tuned C'PI13C) NMR probe with a 9.4-T magnet and perfused with the following substrates given individually or in combination: 10 mM glucose, 2 mM palmitate, and 2.5 mM [3-13C]pyruvate. Glucose was th...

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Intracellular and not intraluminal esterolysis of enalapril in kidney. Studies with the single pass perfused nonfiltering rat kidney.

Two possible sites of renal metabolism exist: intracellular, by enzymes within the peritubular cells, and intraluminal, by ecto-enzymes embedded on the brush border membrane. The esterolysis of enalapril to its dicarboxylate metabolite, enalaprilat, was studied in the isolated perfused, nonfiltering rat kidney preparation (NFK) and compared with that observed for the isolated perfused rat kidne...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1992

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)50572-5